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Article Title: Fasting-induced FGF21 signaling activates hepatic autophagy and lipid degradation via JMJD3 histone demethylase
Article Snippet: Information on antibodies Antibody Company # of catalogue dilutions JMJD3 Santa Cruz Biotechnology sc-130159 1: 5000 KLB Santa Cruz Biotechnology sc-74343 1: 5000 GAPDH Santa Cruz Biotechnology sc-166574 1: 5000 LAMIN A Santa Cruz Biotechnology sc-20680 1: 5000 PPARα Santa Cruz Biotechnology sc-9000 1: 5000 LAMP1 Abcam ab24170 1: 5000 H3K27-me3 Abcam ab6002 1: 5000 H3K9-me3 Abcam ab8898 1: 5000 H3K27-ac Abcam ab4729 1: 5000 histone H3 Abcam ab1791 1: 10000 H3K4-me3 Abcam ab8580 1: 5000 ACTIN Cell Signaling #4970 1: 10000 LC3-I/II Cell Signaling #4108 1: 3000 p62 Cell Signaling #5114 1: 2000 TFEB Cell Signaling #37785 1: 3000 ULK1 Cell Signaling #8054 1: 5000 p-Thr Cell Signaling #9386 1: 5000 EZH2 Cell Signaling #5246 1: 3000 p-S6 Cell Signaling #4858 1: 3000 S6 Cell Signaling #2217 1: 5000 p-ERK Cell Signaling #9101 1: 3000 ERK Cell Signaling #4695 1: 5000 CREB Cell Signaling #9104 1: 3000 FOXO1 Cell Signaling #2880 1: 5000 p-PKA Cell Signaling #4781 1: 5000 PKA Cell Signaling #4782 1: 5000 ATG7 Cell Signaling #2631 1: 3000 KLB R&D Systems AF2619 1: 3000 FGF21 R&D Systems AF3057 1: 3000 LAMP2 Sigma Inc L0668 1: 5000 FLAG Sigma Inc F3165 1: 10000 Supplementary Table 2.
Western Blot:Article Title: Endotoxemia-mediated activation of acetyltransferase P300 impairs insulin signaling in obesity
Article Snippet: .. Name of Antibody Manufacturer Catalog# Species Dilution used P300 Santa Cruz, sc-585 Abcam, ab14984 Rabbit Mouse 1:200 1 ug/ml CBP Santa Cruz, sc-369 Rabbit 1:200 CRTC2 Cell Signaling, 13017 Mouse 1:1000 FoxO1 Cell Signaling, 9454 Rabbit 1:1000 CREB Cell Signaling, 9104 Mouse 1:1000 pPERK Cell Signaling, 3179 Rabbit 1:1000 pIRE1 Abcam, ab48187 Rabbit 1:1000 IRE1 Abcam, ab37073 Rabbit 1 ug/ml β-Actin Santa Cruz, sc-81178 Mouse 1:5000 Tubulin Cell Signaling, 2144 Rabbit 1:1000 Lamin A/C Cell Signaling, 4777 Mouse 1:2000 XBP1 Santa Cruz, sc-7160 Rabbit 1:500 pGSK3 Cell Signaling, 9331 Rabbit 1:1000 pAKT (473) Cell Signaling, 9271 Rabbit 1:1000 GSK3 Cell Signaling, 9315 Rabbit 1:1000 AKT Abcam, ab8805 Rabbit 1:1000 IRS1 Cell Signaling, 3407 Rabbit 1:1000 IRS2 Millipore, MABS15 Mouse 0.5 ug/ml IRb Cell Signaling, 3025 Rabbit 1:1000 p85 Cell Signaling, 4292 Rabbit 1:1000 P110α Cell Signaling, 4255 Rabbit 1:1000 phospho-tyrosine Millipore, 05-321 Mouse 1 ug/ml phospho-serine Millipore, AB1603 Rabbit 1:500 Anti-Acetylated Lysine Cell Signaling, 9814 Rabbit 1:1000 Anti-FLAG Sigma, F3165 Mouse 2 ug/ml Anti-HA Cell Signaling, 3724 Rabbit 1:1000 Anti-ubiquitin Cell Signaling, 3933 Rabbit 1:1000 Anti-phospho-IR (972) Millipore, 07-838 Rabbit 1:1000 Supplementary Table 1 Antibodies used for immunoblots R el at iv e P3 00 m R N A NS 0 1 2 3 4 3 d 1 wk 2 wks 4 wks RD HFD W ei gh t G ai n (g ) * * a b c d 0 50 100 150 200 0 10 20 30 60 90 120 RD HFD B lo od G lu co se (% ba sa l) (min) Pyruvate Tolerance Test (CD14 KO) e Lean ob/ob Cytosol P300 P300 ip P300 α-Tubulin Lamin A/C Input f g LPS DAPI P300 XBP1 DAPI+P300 DAPI+XBP1 P300+XBP1 DAPI+P300+XBP1 Vehicle P300 RD HFD Adipose Actin 250 37 kDa P300 RD HFD Muscle Actin 250 37 kDa 250 250 50 75 50 kDa Supplementary Figure 1 Supplementary Figure 1 LPS treatment changes the cellular localization of P300 in hepatocytes. (a) Body weight gain in mice fed a regular chow diet or a HFD for 1~4 weeks (n=4/group). .. *, p<0.05, paired sample t-test between groups at each time point. (b) Hepatic mRNA levels of P300 from mice fed a regular chow diet or a HFD for 2 weeks (normalized to 36B4 expression levels, n=4). (c, d) P300 protein levels in adipose tissues (c) and muscle (d) from mice fed a regular chow diet or a HFD for 2 weeks (n=4~5/group). (e) Pyruvate tolerance test (4 h fasting, 2 g/kg) in CD14 knockout mice fed a HFD for 2 weeks (n=4/group). (f) Cytoplasmic P300 proteins from lean control and ob/ob mice were immuneprecipitated using P300 antibody (Santa Cruz) and immuoblotted with P300 antibody (abcam).
Activation Assay:Article Title: FoxO1/Rictor axis induces a nongenetic adaptation to ibrutinib via Akt activation in chronic lymphocytic leukemia
Article Snippet: .. FoxO1/Rictor Axis Induces a Non-Genetic Adaptation to Ibrutinib via Akt Activation in Chronic Lymphocytic Leukemia Ondrisova et al. Uncropped original blots from the manuscript Detected protein Manufacturer Catalog number Dilution Rictor Cell Signaling #2114 1:2000 mTOR Cell Signaling #2983 1:2000 pmTOR Cell Signaling #2974 1:1000 FoxO1 Cell Signaling #2880 1:2000 pFoxO1 Cell Signaling #2599 1:1000 Akt Cell Signaling #2920 1:2000 pAktS473 Cell Signaling #4060 1:1000 pAktT308 Cell Signaling #13038 1:1000 BTK Cell Signaling #3533 1:2000 pBTK Cell Signaling #5082 1:1000 GAB1 Cell Signaling #3232 1:2000 GSK3α/β Cell Signaling #5676 1:2000 pGSK3α/β Cell Signaling #9331 1:2000 cMYC Cell Signaling #5605 1:2000 GAPDH Cell Signaling #2118 1:2000 Vinculin Santa Cruz sc73614 1:2000 List of used antibodies pAktS473 tAkt GAPDH tAkt GAPDH pAktS473 Full unedited gels for Figure 1A CLL No. 17 CLL No. 43 tAkt pAktS473 pGSK3αS21/βS9 tGSK3α/β Rictor GAPDH Full unedited gels for Figure 1C GAPDH Rictor GAPDH Rictor Full unedited gels for Figure 2C CLL No. 47 CLL No. 40 Rictor pmTORS2481 mTOR pAktS473 tAkt GAPDH Full unedited gels for Figure 2F Rictor pAktS473 tAkt GAPDH mTOR pmTOR S2481 tGSK3α/β pGSK3αS21/βS9 Full unedited gels for Figure 2H pAktS473 pmTOR S2481 Rictor tAkt mTOR GAPDH pAktS473 tAkt cMYC GAPDH Full unedited gels for Figure 2I Full unedited gels for Figure 2J pAktT308 FoxO1 GAPDH CLL No. 26 FoxO1 GAPDH CLL No. 15 FoxO1 GAPDH CLL No. 45 Full unedited gels for Figure 3C FoxO1 GAPDH pFoxO1T24 Full unedited gels for Figure 3A FoxO1 pAktS473 tAkt GAPDH Rictor Full unedited gels for Figure 4A Rictor FoxO1 tAkt pAktS473 GAPDH Full unedited gels for Figure 4B Rictor pAktS473 tAkt GAPDH tGSK3α/β pGSK3αS21/βS9 Full unedited gels for Figure 4D Full unedited gels for Figure 4E pAktS473 tAkt pmTORS2481 Rictor mTOR vinculin pAktS473 tAkt pmTORS2481 Rictor GAPDH mTOR Full unedited gels for Figure 4F Rictor tAkt cMYC GAPDH pAktS473 Full unedited gels for Figure 4G pAktS473 tAkt FoxO1 GAPDH Rictor Full unedited gels for Figure 5B Full unedited gels for Figure 6A Rictor tAkt GAPDH pAktS473 pAktS473 tAkt GAPDH Full unedited gels for Figure 6C pAktS473 tAkt FoxO1 Rictor GAPDH pAktS473 tAkt FoxO1 Rictor GAPDH pAktS473 tAkt FoxO1 Rictor GAPDH C LL N o . ..
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